2 edition of CD44 is anti-apoptotic in the murine colonic epithelium found in the catalog.
CD44 is anti-apoptotic in the murine colonic epithelium
Written in English
CD44 is a unique adhesion molecule in that it facilitates both cell-cell and cell-matrix interactions. These adhesive interactions are important in maintaining colonic crypt homeostasis which is characterized by migration of epithelial cells from the crypt base to the luminal surface where cell detachment and apoptosis occurs. Dysregulation of this process is a feature of neoplastic transformation and defective apoptosis is evident in colon carcinogenesis. Although dysregulated expression of CD44 occurs consistently during this transformation, how it contributes to carcinogenesis remains unknown. I, therefore, hypothesized that CD44 plays an anti-apoptotic role in the colonic epithelium, in conjunction with other anti-apoptotic proteins.I used a CD44 knockout murine model to investigate the role of CD44 in apoptosis in the colon, and the factors involved in mediating its anti-apoptotic role. I found that CD44 expression was associated with an anti-apoptotic phenotype in the colonic epithelium, at the ultrastructural and molecular level. Supporting evidence in CD44-/- colon included abnormal mitochondrial ultrastructure and activation of caspase 3. Furthermore, I demonstrated the involvement of the mitochondrial pathway of apoptosis in CD44-/- colon as shown by activation of caspase 9 and an imbalance between pro and anti-apoptotic members of the Bcl-2 family. In addition, comparable Ki-67 expression and BrdU labeling in the crypt epithelium demonstrated that CD44 deficiency did not alter proliferation, which works in concert with apoptosis to maintain colonic epithelium homeostasis. Therefore, I concluded that loss of CD44 rendered the colonic epithelium predisposed to apoptosis. I further investigated into this protective role of CD44 using whole mouse irradiation to induce apoptosis in the colonic epithelium. I found that CD44 deficiency was associated with significant increase in apoptotic bodies and enhanced caspase 3 activation. Susceptibility to radiation induced apoptosis in CD44 deficient cells involved the mitochondrial pathway exclusively based on activation of caspase 9 and not 8. Collectively, my study identified an anti-apoptotic role to CD44 in the colonic epithelium. This role is relevant to the biology of colorectal neoplasia as it provides an understanding of the consequences of overexpression of CD44 in colonic adenomas and carcinomas.
|Statement||by Minalini Lakshman.|
|The Physical Object|
|Pagination||xii, 134 leaves.|
|Number of Pages||134|
Described herein are chimeric Newcastle disease viruses comprising a packaged genome comprising a transgene encoding interleukin The chimeric Newcastle disease viruses and compositions thereof are useful in combination with an antagonist of programmed cell death protein 1 (“PD-1”) or a ligand thereof in the treatment of cancer. In particular, described herein are methods for treating. Abciximab was approved in Infliximab (Remicade), approved in , is a murine-human chimeric antibody that targets TNF-alpha (14). This antibody is indicated for chronic use in Crohn's disease and rheumatoid arthritis. Despite the reduction in murine content in these chimerized antibodies, HAMA responses have been reported to/5.
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Infectious Diseases Gastric epithelium Human Cervical epithelium Human Hepatocyte Human Nasopharyngeal Human epithelium Bile duct epithelium Hamster Tumor tissue Human Urinary bladder epithelium Ref. (34) (36) (35) (37) (30,32,33,51) (52) Human (38) Inflammatory Conditions Colon epithelium Mouse (39). Oral Abstracts Oral Abstracts imm__s1_title_Layout 1 Page 1 IMMUNOLOGY ABSTRACTS Immunology The Journal of cells, molecules, systems and technologies Volume , Suppl. 1, October Proceedings of the 9th European Mucosal Immunology Group meeting, October Editor Allan Mowat and Simon Milling Disclaimer: This .
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The present study, using a CDdeficient murine model that lacks a gross phenotype (Schmits et al., ), describes CD44 as being anti-apoptotic in the colonic epithelium and identifies cellular proteins involved in this process. Altered phenotype consistent with apoptosis was observed at the ultrastructural and molecular level in the CD44 Cited by: CD44 negatively regulates apoptosis in murine colonic epithelium via the mitochondrial pathway Article in Experimental and Molecular Pathology 76(3) July with 8 Reads.
Interestingly, among them it has been shown that CD44, whose expression is dysregulated in most types of cancer, plays an important role as shown in murine colonic epithelium  and human colon. Introduction.
CD44, a complex transmembrane glycoprotein, also called Hermes antigen, homing cell adhesion molecule, HUTCH-1, phagocytic glycoprotein-1, lymphocyte-homing receptor, and ECM-III, is encoded by the CD44 gene on chromos1 which consists of 20 exons.2 Transcripts for the CD44 gene undergo complex alternative splicing, which results in many functionally distinct Cited by: Aim.
Programmed cell death is essential both during normal organ development and carcinogenesis. In this study we immunohistochemically analyzed different pathways of cell death in 11 human conceptuses 5thth-weeks old, 10 low and high grade colorectal carcinomas (CRC), and 10 normal colon samples by using markers for apoptosis (caspase-3, AIF, TUNEL), proliferation (Ki) and stemness (Oct-4).Cited by: 3.
Apoptosis Mechanisms. Apoptosis is the orchestration of cell death by highly conserved genes in eukaryotes [10–13] that permits the removal of damaged or unneeded cells from tissue without releasing cellular contents that would otherwise damage surrounding cells or elicit an inflammatory is tightly and carefully regulated in normal circumstances but may be inappropriately Cited by: The life cycle of an intestinal epithelial cell is terminated by apoptosis and/or cell shedding.
Apoptotic deletion of epithelial cells from the intact intestinal mucosa is not accompanied by detectable inflammatory response or loss of barrier function. But increased permeability of the epithelial barrier and increased apoptotic rates of epithelial cells have been reported for patients Cited by: Nowadays it is reported that, similarly to other solid tumors, colorectal cancer is sustained by a rare subset of cancer stem–like cells (CSCs), which survive conventional anticancer treatments, thanks to efficient mechanisms allowing escape from apoptosis, triggering tumor recurrence.
To improve patient outcomes, conventional anticancer therapies have to be replaced with specific approaches Cited by: Caspase-9 is involved in the intrinsic apoptotic pathway and suggested to play a role as a tumor suppressor. Little is known about the mechanisms governing caspase-9 expression, but post-transcriptional pre-mRNA processing generates 2 splice variants from the caspase-9 gene, pro-apoptotic caspase-9a and anti-apoptotic caspase-9b.
Lakshman M, Subramaniam V, Wong S, Jothy S () CD44 promotes resistance to apoptosis in murine colonic epithelium. J Cell Physiol – PubMed Google Scholar Cited by: 2. It is widely accepted by the scientific community that cancer, including colon cancer, is a “stem cell disease”. Until a few years ago, common opinion was that all neoplastic cells within a tumor contained tumorigenic growth capacity, but recent evidences hint to the possibility that such a feature is confined to a small subset of cancer-initiating cells, also called cancer stem cells (CSCs).Cited by: 9.
In addition to reducing inflammation, ATB suppressed expression of a number of proinflammatory cytokines (IL-1, TNF, IL) and markedly reduced granulocyte infiltration into the colonic tissue. Another class of therapeutics we have focused on is nonsteroidal anti-inflammatory drugs (NSAIDs).
Stem cell markers are genes and their protein products used by scientists to isolate and identify stem cells can also be identified by functional assays. Below is a list of genes/protein products that can be used to identify various types of stem cells, or functional assays that do the same.
The initial version of the list below was obtained by mining the PubMed database as described in. mains, whether we can expect anti-apoptotic ef-fects of CD14 in these macrophages in situ; it also remains to be elucidated whether the longer life expectancy of macrophages in situ is determined by resistance to stimuli that elicit apoptosis of blood monocytes in vitro (Kiener et al.
This is very important because in situ macrophages. The link between inflammation and cancer was suggested a long time ago and it has become a major part of current cancer research. Even analyzing the biophysical parameters or preferences of inflammation is a growing research field and it will probably become the future focus of physics of cancer.
Book Description: Acute appendicitis is one of the most common surgical abdominal emergencies worldwide, with a lifetime risk of % for males and % for females. This compilation discusses how while non-operative treatment of appendicitis has been proposed as an alternative to surgery, appendicectomy remains the mainstay of treatment in.
Cancer stem cells (CSCs) are cancer cells (found within tumors or hematological cancers) that possess characteristics associated with normal stem cells, specifically the ability to give rise to all cell types found in a particular cancer sample.
CSCs are therefore tumorigenic (tumor-forming), perhaps in contrast to other non-tumorigenic cancer cells.
CSCs may generate tumors through the stem.Full text of "Stem Cells And Cancer Stem Cells" See other formats.The intestinal epithelium is the most rapidly self-renewing tissue of the body: Cells have a life cycle of days.
Lymphoid cells and lymphatic nodules are in the submucosa.